C# Class Bio.Variant.VariantCaller

This class takes alignments and generates a list of variants.

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Méthodes publiques

Méthode	Description
CallVariants ( IPairwiseSequenceAlignment aln ) : List	Given a pairwise sequence alignment, call variants, producing a list of SNPs and Indels found in the alignment. This method will first left-align all variants before calling to be consistent with other software. The
LeftAlignIndelsAndCallVariants ( IPairwiseSequenceAlignment aln, bool callVariants = true ) : List>.Tuple	Given two byte arrays representing a pairwise alignment, shift them so that all deletions start as early as possible. For example: `TTTTAAAATTTT -> Converts to -> TTTTAAAATTTT TTTTAA--TTTT TTTT--AATTTT` This function takes a IPairwiseSequenceAlignment and assumes that the first sequence is the reference and second sequence is the query. It returns a new Pairwise sequence alignment with all of the indels left aligned as well as a list of variants.

Private Methods

Méthode	Description
CallVariants ( byte refSeq, Bio.Variant.BPandQV querySeq, bool originallyReverseComplemented ) : List	Calls the variants. Should only be used internally as assumptions are made that the alignments are left-aligned and fulfill certain criteria.
determineHomoPolymerLength ( int pos, byte refSeq ) : char>.Tuple	Returns the length of the homopolymer
getBases ( Bio.Variant.BPandQV array, int position, int length ) : string	Gets the bases for a length of the sequence as a string.
getBases ( byte array, int position, int length ) : string	Converts a subset of bases in the array into a string.

Method Details

CallVariants() public static méthode

Given a pairwise sequence alignment, call variants, producing a list of SNPs and Indels found in the alignment. This method will first left-align all variants before calling to be consistent with other software. The

public static CallVariants ( IPairwiseSequenceAlignment aln ) : List
aln	IPairwiseSequenceAlignment	The Pairwise alignment to call variants with.
Résultat	List

LeftAlignIndelsAndCallVariants() public static méthode

Given two byte arrays representing a pairwise alignment, shift them so that all deletions start as early as possible. For example: TTTTAAAATTTT -> Converts to -> TTTTAAAATTTT TTTTAA--TTTT TTTT--AATTTT This function takes a IPairwiseSequenceAlignment and assumes that the first sequence is the reference and second sequence is the query. It returns a new Pairwise sequence alignment with all of the indels left aligned as well as a list of variants.

public static LeftAlignIndelsAndCallVariants ( IPairwiseSequenceAlignment aln, bool callVariants = true ) : List>.Tuple
aln	IPairwiseSequenceAlignment	Aln. The second sequence should be of type QualitativeSequence or Sequence
callVariants	bool	callVariants. If true, it will call variants, otherwise the second half of tuple will be null.
Résultat	List>.Tuple